Characteristics of Three NADH Dehydrogenases in Bacteria
NDH-1 NDH-2 Na+-NDH
Ion-pump H+ (and Na+?) not a pump Na+
Cofactors one noncovalent FMN
2 [2Fe-2S]
6 - 8 [4Fe-4S]
See Table
one noncovalent FAD one noncovalent FAD (subunit F)
one noncovalent riboflavin (subunit ?)
two covalent FMN (subunits B and C)
one [2Fe-2S] (subunit F)
Subunit Composition 13-14 1 6
Inhibitors Rotenone
Piericidin A
Capsaicin
Acetogenins
Pyridaben
Fenpyroximate
Flavone (?) Ag+
HQNO
Korormicin
Organisms Paracoccus denitrificans
Rhodobacter capsulatus
Escherichia coli
Synechocystis PCC6803
Thermus thermophilus
Klebsiella pneumoniae
many gram-positive bacteria
many archaea
Vibrio
Haemophilus influenzae
Escherichia coli
Vibrio
Haemophilus influenzae
Pseudomonas aeruginosa
Klebsiella pneumoniae